ABSTRACT
Abstract Objective Despite some knowledge gaps in scientific evidence, MgCl2 is largely used for pain relief in musculoskeletal diseases. Mg salts were shown to provide analgesia postoperatively in orthopedic surgery and low Mg levels were linked to arthritis development and severity. We determined the anti-inflammatory activity of MgCl2 in an acute arthritis model. Methods Mice received 0.1 mg/25μL Zymosan (Zy) or saline into the knees. Joint pain was evaluated using von Frey test; cell influx, and interleukin (IL)-1 level were assessed in joint lavage at 6 h. Synovia were excised for histopathology and analysis of immunoexpression of nuclear factor kappa B (NFκB) and tumor necrosis factor (TNF)-α. Groups (n = 6/group) received either 90 mg/kg MgCl2/100 μL or saline per os (systemic) or 500 μg/25 μL MgCl2 or saline intra-articularly (i.a.) 30 min prior to Zy. Results MgCl2 given either systemically or locally significantly reduced cell influx (p = 0.0012 and p = 0.0269, respectively), pain (p = 0.0005 and p = 0.0038, respectively), and intra-articular IL-1 level (p = 0.0391), as compared to saline. Systemic MgCl2 significantly decreased NFκB (p < 0.05) immmunoexpression, as compared to saline. Conclusion MgCl2 given systemically or locally displayed anti-inflammatory activity in a severe acute arthritis model reducing cell influx, pain, and cytokine release. MgCl2 operates at least partially via inhibiting NFκB activation. This is the first in vivo demonstration that MgCl2 decreases cytokine release in arthritis, prompting reduction of inflammation and pain relief.
ABSTRACT
Sleep is essential for the physical, psychological, and social well-being of individuals. Its poor quality/quantity may lead to functional impairment in the performance of social activities and interpersonal relationships. In this context, studies have identified poor sleep quality in university students, especially those in healthcare. Despite the literature pointing out that nursing students do not sleep well, factors related to employment or daily commuting are not unanimous. Thus, the objective of the study is to evaluate the sleep quality of nursing students and its association with socioeconomic characteristics, life habits, academic, and extracurricular activities. This is a cross-sectional, descriptive study with quantitative analysis, carried out with 124 Nursing students from a private Higher Education Institution in Ceará. A questionnaire was used with socioeconomic variables, life-style habits, academic and extracurricular activities, and the Pittsburgh Sleep Quality Index. Descriptive and inferential statistical analyses were performed. The results of this study showed a predominance of poor sleep quality for 73 (59%) students and the presence of sleeping disorders for 38 (31%) of the participants. The variables associated with this result were the daily intercity commute (to-and-fro) to attend an undergraduate class or to have a concomitant job. Meanwhile, the other academic, extracurricular, and lifestyle variables did not have a statistically significant association with sleep quality. Therefore, it is necessary to adopt actions aimed at good sleep hygiene, for better use of the available time for sleep, better academic performance, and quality of life.
O sono é essencial para o bem-estar físico, psíquico e social dos indivíduos. Sua qualidade/quantidade ruim poderá levar a prejuízo funcional no desempenho dos papéis sociais e nas relações interpessoais. Nesse contexto, estudos comprovam uma qualidade do sono ruim em universitários, especialmente os da área da saúde. Apesar da literatura apontar que universitários de enfermagem são maus dormidores, fatores relacionados como vínculo empregatício ou deslocamento intermunicipal diário não são unânimes. Assim, o objetivo do estudo é avaliar a qualidade do sono de estudantes de enfermagem e sua associação com características socioeconômicas, hábitos de vida, atividades acadêmicas e extracurriculares. Trata-se de um estudo transversal, descritivo, com análise quantitativa, realizado com 124 estudantes de Enfermagem de uma Instituição de Ensino Superior privada cearense. Empregou-se questionário com variáveis socioeconômicas, hábitos de vida, atividades acadêmicas e extracurriculares, e o Índice da Qualidade do Sono de Pittsburgh. Foi realizada análise estatística descritiva e inferencial. Os resultados deste estudo demonstraram predominância de qualidade do sono ruim para 73 (59%) estudantes e presença de distúrbios do sono para 38 (31%) dos participantes. As variáveis associadas a esse resultado foram o deslocamento intermunicipal diário (movimento pendular) para cursar a graduação ou possuir vínculo empregatício concomitante. Enquanto, as demais variáveis acadêmicas, extracurriculares e de hábitos de vida não tiveram associação estatisticamente significante com a qualidade do sono. Logo, é necessária a adoção de ações visando a boa higiene do sono, para melhor aproveitamento do período disponível para o sono, melhor desempenho acadêmico e qualidade de vida
ABSTRACT
O sono é essencial para o bem-estar físico, psíquico e social dos indivíduos. Sua qualidade/quantidade ruim poderá levar a prejuízo funcional no desempenho dos papéis sociais e nas relações interpessoais. Nesse contexto, estudos comprovam uma qualidade do sono ruim em universitários, especialmente os da área da saúde. Apesar da literatura apontar que universitários de enfermagem são maus dormidores, fatores relacionados como vínculo empregatício ou deslocamento intermunicipal diário não são unânimes. Assim, o objetivo do estudo é avaliar a qualidade do sono de estudantes de enfermagem e sua associação com características socioeconômicas, hábitos de vida, atividades acadêmicas e extracurriculares. Trata-se de um estudo transversal, descritivo, com análise quantitativa, realizado com 124 estudantes de Enfermagem de uma Instituição de Ensino Superior privada cearense. Empregou-se questionário com variáveis socioeconômicas, hábitos de vida, atividades acadêmicas e extracurriculares, e o Índice da Qualidade do Sono de Pittsburgh. Foi realizada análise estatística descritiva e inferencial. Os resultados deste estudo demonstraram predominância de qualidade do sono ruim para 73 (59%) estudantes e presença de distúrbios do sono para 38 (31%) dos participantes. As variáveis associadas a esse resultado foram o deslocamento intermunicipal diário (movimento pendular) para cursar a graduação ou possuir vínculo empregatício concomitante. Enquanto, as demais variáveis acadêmicas, extracurriculares e de hábitos de vida não tiveram associação estatisticamente significante com a qualidade do sono. Logo, é necessária a adoção de ações visando a boa higiene do sono, para melhor aproveitamento do período disponível para o sono, melhor desempenho acadêmico e qualidade de vida.
Sleep is essential for the physical, psychological, and social well-being of individuals. Its poor quality/quantity may lead to functional impairment in the performance of social activities and interpersonal relationships. In this context, studies have identified poor sleep quality in university students, especially those in healthcare. Despite the literature pointing out that nursing students do not sleep well, factors related to employment or daily commuting are not unanimous. Thus, the objective of the study is to evaluate the sleep quality of nursing students and its association with socioeconomic characteristics, life habits, academic, and extracurricular activities. This is a cross-sectional, descriptive study with quantitative analysis, carried out with 124 Nursing students from a private Higher Education Institution in Ceará. A questionnaire was used with socioeconomic variables, life-style habits, academic and extracurricular activities, and the Pittsburgh Sleep Quality Index. Descriptive and inferential statistical analyses were performed. The results of this study showed a predominance of poor sleep quality for 73 (59%) students and the presence of sleeping disorders for 38 (31%) of the participants. The variables associated with this result were the daily intercity commute (to-and-fro) to attend an undergraduate class or to have a concomitant job. Meanwhile, the other academic, extracurricular, and lifestyle variables did not have a statistically significant association with sleep quality. Therefore, it is necessary to adopt actions aimed at good sleep hygiene, for better use of the available time for sleep, better academic performance, and quality of life.
ABSTRACT
Abstract Background: Injection of Hylan G-F20 (HY) into joints may provoke local flares, which mechanisms may involve reaction to protein contaminants. We have previously developed a protein-free saline-soluble galactomannan derived from guar gum (GM) that displays both analgesia and chondroprotection in experimental osteoarthritis (OA). We now demonstrate that both GM and Hylan G-F20 (HY) promote mild synovitis with cytokine release after intra-articular injection. Methods: Mice received 100 μg/25 μL GM or HY or saline into the knees. Joint pain was evaluated using von Frey test; cell influx, interleukin (IL)-1, IL-6, and CXCL-1 (pg/mL) levels were assessed in joint lavage at 6 h. Synovia were excised for histopathology. Results: Neither GM nor HY after being given into mice knee joints induced pain albeit promoting mild cell influx into joint washings as well as mild synovitis at histology, with no damage to the underlying cartilage. HY but not GM promoted IL-1 release into mice joints. Both compounds induced IL-6 and CXCL-1 release. Conclusion: Intra-articular injection of HY or GM promote acute transient synovitis whilst not provoking detectable significant joint damage. Local administration of these polysaccharides induces acute intra-articular release of inflammatory cytokines, which may account for joint flares following viscosupplementation.(AU)